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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
02/01/2017 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTILLO, A.; GAIERO, P.; LÓPEZ CARRO, B.; VILARO, F. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; P. GAIERO, Universidad de la República (UdelaR)/ Facultad de Agronomía; B. LÓPEZ CARRO, SECIF-IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Plant Tissue Culture and Biotechnology, 2016, v. 26 (2): 159-173. |
DOI : |
10.3329/ptcb.v26i2.30566 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
Palabras claves : |
ANTHER CULTURE; DOUBLE HAPLOID; FLOW CYTOMETRY; HAPLOID. |
Thesagro : |
GERMOPLASMA; PAPA; SOLANUM COMMERSONII. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13501/1/30566-Article-Text-109802-1-10-20161210.pdf
https://www.banglajol.info/index.php/PTCB/article/view/30566
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Marc : |
LEADER 01724naa a2200253 a 4500 001 1056407 005 2019-10-15 008 2016 bl uuuu u00u1 u #d 024 7 $a10.3329/ptcb.v26i2.30566$2DOI 100 1 $aCASTILLO, A. 245 $aGametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.$h[electronic resource] 260 $c2016 520 $aABSTRACT. The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. 650 $aGERMOPLASMA 650 $aPAPA 650 $aSOLANUM COMMERSONII 653 $aANTHER CULTURE 653 $aDOUBLE HAPLOID 653 $aFLOW CYTOMETRY 653 $aHAPLOID 700 1 $aGAIERO, P. 700 1 $aLÓPEZ CARRO, B. 700 1 $aVILARO, F. 773 $tPlant Tissue Culture and Biotechnology, 2016$gv. 26 (2): 159-173.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
02/10/2014 |
Actualizado : |
29/03/2017 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
REBUFFO, M.; MONZA, J.; CASTILLO, A.; SANJUÁN, J.; REYNO, R.; BATISTA, L.; QUERO, G.; CUITIÑO, M.J. |
Afiliación : |
MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARIA JOSE CUITIÑO DE VEGA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Multidisciplinary approaches to improve forage legume species for stressing environments in South America. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
In: INTERNATIONAL SYMPOSIUM ON THE MOLECULAR BREEDING OF FORAGE AND TURF, 7., 2012, Salt Lake City, UT, US. Proceedings: other invited oral presentation abstracts. Salt Lake City, UT: MBFT, 2012. |
Páginas : |
p. 62. |
ISBN : |
978-1-4675-4762-8 |
Idioma : |
Inglés |
Palabras claves : |
FIJACIÓN BIOLÓGICA DEL NITRÓGENO; RIZOBIOLOGÍA; SIMBIONTES. |
Thesagro : |
DIVERSIDAD GENÉTICA; LEGUMINOSAS FORRAJERAS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 00944nam a2200265 a 4500 001 1050813 005 2017-03-29 008 2012 bl uuuu u01u1 u #d 020 $a978-1-4675-4762-8 100 1 $aREBUFFO, M. 245 $aMultidisciplinary approaches to improve forage legume species for stressing environments in South America.$h[electronic resource] 260 $aIn: INTERNATIONAL SYMPOSIUM ON THE MOLECULAR BREEDING OF FORAGE AND TURF, 7., 2012, Salt Lake City, UT, US. Proceedings: other invited oral presentation abstracts. Salt Lake City, UT: MBFT$c2012 300 $ap. 62. 650 $aDIVERSIDAD GENÉTICA 650 $aLEGUMINOSAS FORRAJERAS 653 $aFIJACIÓN BIOLÓGICA DEL NITRÓGENO 653 $aRIZOBIOLOGÍA 653 $aSIMBIONTES 700 1 $aMONZA, J. 700 1 $aCASTILLO, A. 700 1 $aSANJUÁN, J. 700 1 $aREYNO, R. 700 1 $aBATISTA, L. 700 1 $aQUERO, G. 700 1 $aCUITIÑO, M.J.
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